Fluorescence Lifetime Imaging (FLIM) produces an image based on the differences in the excited state decay rate from a fluorescent sample. The signal-to-noise ratio will be determined in part by the relative strength of the autofluorescence compared to the fluorescence of the exogenous probe. Fluorescence Microscopy The CAF Fluorescence Microscopy Unit at Stellenbosch University in the Western Cape houses fluorescence -based instruments for . Fluorescence is a molecular phenomenon in which a substance radiates light energy almost instantaneously upon being struck with light from another source. Both fluorescence and phosphorescence are based on the ability of a substance to absorb light and emit light of a longer wavelength and therefore lower energy.
Fluorescence techniques were designed to observe and analyze the composition of samples, as well as interactions between various . The FLuorescence EXplorer (FLEX) mission will provide global maps of vegetation fluorescence that can reflect photosynthetic activity and plant health and . A fluorescence microscope is much the same as a conventional light microscope with added features to enhance its capabilities.
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